/ Product · Cloning Design
Design your cloning strategy in minutes.
Six guided wizards covering every major cloning method — Gibson Assembly, Golden Gate, Restriction, Gateway, TOPO, and site-directed mutagenesis. Each one produces a visual node graph, automatic primers, and a printable protocol.
Cloning methods
01
Gibson Assembly
Design multi-fragment assemblies with automatic overlap calculation and primer generation.
02
Golden Gate
Build type IIS restriction enzyme assemblies with fusion-site optimisation to minimise scar sequences.
03
Restriction Cloning
Select enzyme pairs, visualise compatible ends, and get a step-by-step digest and ligation protocol.
04
Gateway Cloning
Design BP and LR recombination strategies with attB site insertion and destination vector selection.
05
TOPO Cloning
Configure blunt-end or 3′-overhang TOPO reactions with compatible vector and insert checks.
06
Site-Directed Mutagenesis
Introduce point mutations, insertions, or deletions with mutagenic primer design and protocol output.
Built-in tools
01
Visual node graph
Every cloning strategy is rendered as an interactive node graph — drag fragments, swap vectors, and see how the final construct updates in real time.
02
Automatic primer design
Enter your insert and vector sequences and Talindrew designs the primers, including overhangs, annealing temperatures, and Tm calculations.
03
Protocol export
Export a ready-to-run wet-lab protocol as PDF or Markdown, complete with reagent volumes, thermocycler conditions, and troubleshooting notes.
04
Sequence verification preview
Preview the final construct sequence before you ever pick up a pipette. Confirm reading frame, promoter orientation, and insert direction.
Ready to clone smarter?
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